Microbiological Testing Individual Packed Membrane Filter

9-425 Autosampler Vial & Cap & Septa

Integrated Microbiological Filtration System

Modular Microbial Filtration System

HPLC Column

HPLC Columns are based on ultra-pure (purity > 99.999%) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. column is the best choice for method development, owing to complete bonding chemistries and stable performance.

product details

Applications

Material

Ultra-pure spherical porous silica, purity > 99.999%	Wide pH range: 1.5-10
Unique bonding chemistry and endcapping technology	Long column life time
High efficiency: theoretical plate > 80000/m	Exceptional lot-to-lot reproducibility
Excellent peak symmetry: tailing factor=0.95~1.05	Complete bonding chemistries with different selectivity

HPLC Column Packing Materials Pictures below show size uniformity and surface smoothness of the packing particles, characteristics that enable more uniform packing with less channeling effect, resulting in lower back pressure and higher column efficiency. welch-chromatography-hplc-catalog-2025-1-11f

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C18 HPLC Column

C18 is the most commonly used column in the market. It can substitute Waters Symmetry C18, Agilent Zorbax XDB C18, Phenomenex Luna C18, Supelcosil LC-18-DB, YMC ODS-AM, Alltima C18, GL, Inertsil ODS-2 etc. C18 has high theoretical plates and peak capacity, so it’s suitable for analysis of complex samples.

C18	Structural Formula
PH Range	1.5-10.0	Carbon Loading（%）	17（120A），8（300A）
Particle Size	3μm，5μm，10μm	USP List	L1
Surface Area（m²/g）	320（120A），90（300A）	Endcapped	Yes

C8 HPLC Column

C8 phase is less retentive than C18 phase, useful for strong hydrophobic compounds that are too strongly retained on C18 phase, and for LC m /MS applications, where long retention is not desired. When separating neutral or other highly retained compounds, C8 can save analytical time. However, when separating polar compounds, -C8 column provides different selectivity than does C18 column.

C18	Structural Formula
PH Range	1.5-10.0	Carbon Loading（%）	17（120A），8（300A）
Particle Size	3μm，5μm，10μm	USP List	L7
Surface Area（m²/g）	320（120A），90（300A）	Endcapped	Yes

Phenyl HPLC Column

Phenyl phase is less retentive than conventional C18 or C8 phases, but more retentive than standard cyano phase. Due to their ability to participate in π-π interactions, Phenyl columns may actually be more retentive than C18 or C8 columns towards certain polar aromatic compounds, depending on running conditions. The selectivity for highly polar aromatics, which are poorly retained on alkyl-bonded phases, together with reduced retentivity towards non-polar compounds, make Phenyl an excellent choice for the analysis of complex mixtures of polar and non-polar analytes. Additionally, this bonding phase boasts high surface coverage and exhaustive double end-capping, leading to better performance.

Phenyl	Structural Formula
PH Range	1.5-10.0	Carbon Loading（%）	12（120A），4（300A）
Particle Size	3μm，5μm，10μm	USP List	L11
Surface Area（m²/g）	320（120A），90（300A）	Endcapped	Yes

C4 HPLC Column

1.suitable for separation of bio-samples

2.Strong retention for hydrophobic and polar compounds •

3.Column packing of 300Å big pore size particles is appropriate for separation of peptide and protein samples with sharp peak shape

4.Mini bore column can be used for LC/MS(/MS)

C4	Structural Formula
PH Range	1.5-10.0	Carbon Loading（%）	8（120A），3（300A）
Particle Size	3μm，5μm，10μm	USP List	L26
Surface Area（m²/g）	320（120A），90（300A）	Endcapped	Yes

C1 HPLC Column

1.Lowest hydrophobicity among reversed phases

2.Intermediate polarity between normal phase silica and other alkyl bonded reversed phase

3.Alternative selectivity to C18 phase

C1	Structural Formula
PH Range	1.5-10.0	Carbon Loading（%）	4（120A）
Particle Size	5μm	USP List	L13
Surface Area（m²/g）	320（120A）	Endcapped	Yes

C4 HPLC Column

1.suitable for separation of bio-samples

2.Strong retention for hydrophobic and polar compounds •

3.Column packing of 300Å big pore size particles is appropriate for separation of peptide and protein samples with sharp peak shape

4.Mini bore column can be used for LC/MS(/MS)

C4	Structural Formula
PH Range	1.5-10.0	Carbon Loading（%）	8（120A），3（300A）
Particle Size	3μm，5μm，10μm	USP List	L26
Surface Area（m²/g）	320（120A），90（300A）	Endcapped	Yes

CN HPLC Column

CN column can be used in either reversed or normal phase. Reversed phase CN column has special selectivity for polar compounds, and due to its low hydrophobicity, elution of hydrophobic molecules is fast. Furthermore, CN column shows perfect peak shape for strong basic analytes (including quaternary ammonium salts). Polarity of CN column is the strongest among all reversed columns. It is a good choice for compounds that are strongly retained on standard reversed columns. Normal phase CN column can replace SiO2 column. Equilibrium of normal phase column is fast, and the silica surface activity is better than that of silica column. To prolong column life time, alternation between reversed phase and normal phase uses should be avoided. While CN column can be used in either reversed or normal phase, elution sequence is different in different separation mode.

1.Can be used in either reversed or normal phases

2.Stable bonding chemistry and excellent surface coverage

3.Low hydrophobicity, unique selectivity

CN	Structural Formula
PH Range	1.5-9.0	Carbon Loading（%）	7（120A)
Particle Size	3μm，5μm，10μm	USP List	L10
Surface Area（m²/g）	320（120A）	Endcapped	Yes

SiO2 HPLC Column

SiO2 column uses ultra-high purity type B silica particles with no metal contents. SiO2 column can separate strong hydrophilic compounds in high concentration organic solvent in reversed phase. Good result can be obtained for the analysis of polar compounds which are prone to peak tailing in reversed phase.

SiO2	Structural Formula
PH Range	2.0-8.0	Carbon Loading（%）	N/A
Particle Size	3μm，5μm，10μm	USP List	L3
Surface Area（m²/g）	320（120A），90（300A）	Endcapped	No

Diol HPLC Column

Diol Column is based on ultra-pure porous spherical silica bonded with 1,2-dihydroxypropyl functional group silica Diol is used in normal phase mostly, suitable for separation of peptides, proteins, polar molecules, and organic acids and its polymers. Like bare silica, Diol has the ability to form hydrogen bonds and is capable of separating structure isomers. Since most of its surface is covered with organic functions, Diol absorbs less water, which leads to more reproducible activity. It is also the sorbent of choice when working in normal phase in the presence of water. It has a different selectivity than bare silica gel, and slight modification in the composition of solvent mixture may be necessary to obtain a similar retention. Diol column is more stable than traditional normal phase columns, such as NH2, SiO2. Compared with NH2/SiO2 column, Diol column is not sensitive to water. Diol column can also be used in reversed phase analysis.

1.More stable than traditional normal phase columns, such as Silica, Amine •

2.Can be used in reversed phase analysis •

3.Similar polarity to Amine •

4.Good selectivity without excessive retention •

5.Improved peak shape compared to bare silica

Diol	Structural Formula
PH Range	2.0-8.0	Carbon Loading（%）	2.5(120A)
Particle Size	3μm，5μm，10μm	USP List	L20
Surface Area（m²/g）	320（120A）	Endcapped	No

NH2 HPLC Column

NH2 column is based on propyl-amino silane, mostly used in normal phase, but can also be used in reversed phase.

1.Used in normal phase for weak anion-exchange, and in reversed-phase HPLC for polar compounds

2.For applications in aggressive normal phase mode with aqueous eluent •

3.Vitamins A and D can be separated in the normal-phase mode •

4.Carbohydrates and sugars can be separated in the reversed-phase mode

NH2	Structural Formula
PH Range	2.0-8.0	Carbon Loading（%）	4(120A)
Particle Size	3μm，5μm，10μm	USP List	L8
Surface Area（m²/g）	320（120A）	Endcapped	No

Ion Exchange HPLC Column

Ion Exchange Column (SAX&SCX) ion exchange columns are available for both SAX and SCX columns. The SCX/SAX columns are silica based with high resolution and high efficiency. SAX is a polar bonded phase, consisting of an ammonium-functionalized silane, while SCX is a classical strong cation exchange, consisting of a covalently bonded aromatic sulfonic acid moiety.

1.Organic modifiers such as acetonitrile and methanol may be used with SAX and SCX columns, within organic/buffer solubility constraints

2.Retention can be controlled by varying pH, ionic strength and organic modifier content.

SAX	Structural Formula
PH Range	2.0-8.0	Carbon Loading（%）	7.5（120A）1.5(300A)
Particle Size	3μm，5μm，10μm	USP List	L14
Surface Area（m²/g）	320（120A），90（300A）	Endcapped	No
SCX	Structural Formula
PH Range	2.0-8.0	Carbon Loading（%）	12（120A）5(300A)
Particle Size	3μm，5μm，10μm	USP List	L9
Surface Area（m²/g）	320（120A）	Endcapped	No

C30 HPLC Column

C30 Carotenoids is a broad class of natural products, of which over 600 types have been found so far, including compounds of different carbon chain length, such as C40, C50 and C30 etc. They are well known to have many biological functions, including cancer prevention and treatment functions. Compared to classical C18 stationary phases, the C30 phase is much more hydrophobic and retaining. Even when pure organic eluent is applied, many sample solutes, such as carotenoids, are able to retain. C30 is designed for the separation of geometric isomers, polar carotenes, polar and nonpolar xanthophylls, steroids, retinols and fat-soluble vitamins (A, D, K and E).

1.Polymeric C30 alkyl chains

2.Very lipophilic

3.Exceptional selectivity pattern for geometric isomers

C30	Structural Formula
PH Range	1.5-10.0	Carbon Loading（%）	22(120A)
Particle Size	3μm，5μm，10μm	USP List	L62
Surface Area（m²/g）	320（120A）	Endcapped	Yes

Syringeless filter vials

Chromfil™ Syringe Filter

Filter Monitor 47mm

Microbiological Testing Individual Packed Membrane Filter

9-425 Autosampler Vial & Cap & Septa

Integrated Microbiological Filtration System

Modular Microbial Filtration System

MKFIL Filter Monitor

Filter monitor 56mm

Automatic Membrane Dispenser

HPLC Column

Applications

Material

C18 HPLC Column

C8 HPLC Column

Phenyl HPLC Column

C4 HPLC Column

C1 HPLC Column

C4 HPLC Column

CN HPLC Column

SiO2 HPLC Column

Diol HPLC Column

NH2 HPLC Column

Ion Exchange HPLC Column

C30 HPLC Column

HPLC Syringes