Ubungakanani bekholamu buquka ubungakanani be-1, 3, 6, kunye ne-10 ml, njl.
Inkqubo yethu yokuvelisa inciphisa ukuguquguquka kwaye iphucula iinkqubo zokubuyisela kunye nokucoca
1.C18 I-Octadecyl Endcapped
2.C18 Ukuphela kwe-Octadecy
3.C8 Octyl
4.CN Cyanopropyl
5. I-PSA Primary-Secondary Amine
6.NH2 Aminopropyl
7. I-SAX Strong Anion Exchange
8. I-SCX Strong Cation Exchange
Iikholamu zeSilica base solid-phase extraction (SPE) zizixhobo ezibalulekileyo kwicandelo lekhemistri yohlalutyo, ezisetyenziselwa ukucoca kunye nokukhupha iikhompawundi ezijoliswe kuzo kwi-matrices zesampulu ezintsonkothileyo. Ezi kholamu ziyilwe ngesigaba esisisigxina esisekelwe kwi-silica, esibonelela ngokukhetha okuphezulu kunye nokusebenza kakuhle kwinkqubo yokwahlulahlula.
Iikholamu zethu ze-SPE zesiseko se-silica ziyafumaneka kwiikholamu ezahlukeneyo, kuquka ubukhulu be-1, 3, 6, kunye ne-10 ml, zilungele iimfuno ezahlukeneyo zokusetyenziswa. Inkqubo yokuvelisa esetyenziswa kwezi kholamu yenzelwe ngobuchule ukunciphisa ukuguquguquka nokuphucula iinkqubo zokubuyisela kunye nokucoca, ukuqinisekisa ukusebenza okuzinzileyo nokuthembekileyo.
Isigaba esimiyo seekholamu zethu ze-silica base SPE sifumaneka kwiindidi ezahlukeneyo zeekhemistri, nganye ilungiselelwe iimfuno ezithile zokwahlulwa. Ezi ziquka i-C18 endcapped octadecyl, i-C18 unendcapped octadecyl, i-C8 octyl, i-CN cyanopropyl, i-PSA primary-secondary amine, i-NH2 aminopropyl, i-SAX strong anion exchange, kunye ne-SCX strong cation exchange. Olu luhlu lwahlukeneyo lweekhemistri luvumela ukukhutshwa kunye nokucocwa ngokufanelekileyo kweentlobo ngeentlobo zee-analytes, okwenza iikholamu zibe nobuchule kwaye zilungelelaniswe nemingeni eyahlukeneyo yohlalutyo.
Umzekelo, isigaba se-octadecyl esifakwe i-C18 endcapped sifanelekile ekugcineni nasekuhlukaniseni iikhompawundi ezingezizo ze-polar kunye ne-polar ephakathi, ngelixa isigaba se-CN cyanopropyl sinika ukhetho oluphuculweyo lweekhompawundi ze-polar. Isigaba se-amine se-PSA esiphambili-sesibini sifanelekile ekukhupheni iikhompawundi ze-acidic, kwaye isigaba se-SAX kunye ne-SCX senzelwe ngokukodwa ukusetyenziswa kwe-ion exchange.
Ukongeza ekukhetheni kwazo okwahlukeneyo, iikholamu zethu ze-SPE zesiseko se-silica zaziwa ngomthamo wazo ophezulu wokulayisha iisampulu, ukuphinda-phinda okugqwesileyo, kunye namanqanaba aphantsi okubopha okungangqalanga, okuqinisekisa iziphumo ezichanekileyo nezithembekileyo. Nokuba zisetyenziswa kuhlalutyo lwezonyango, lokusingqongileyo, lokutya, okanye lwe-forensic, ezi kholamu zibonelela ngokusebenza okuzinzileyo nokuqinileyo, ukuhlangabezana neemfuno zeelabhoratri zohlalutyo zanamhlanje.
Ukuqukumbela, iikholamu ze-SPE zesiseko se-silica zizixhobo ezibalulekileyo zokulungiselela isampuli kwikhemistri yohlalutyo, kwaye uluhlu lwethu lweekholamu, kunye neekhemistri zazo ezahlukeneyo kunye nokusebenza okuthembekileyo, zenzelwe ukuhlangabezana neemfuno ezahlukeneyo zezazinzulu zohlalutyo. Ngenxa yokukhetha kwazo okuphezulu, ukusebenza kakuhle, kunye nomthamo wazo, ezi kholamu zidlala indima ebalulekileyo ekukhutshweni nasekucocweni ngempumelelo kweekhompawundi ezijoliswe kuzo kwiimatrices zesampuli ezintsonkothileyo.
**Isihloko: Ukuvula amandla eekholamu ze-SPE ezisekelwe kwi-silica ukuze kulungiselelwe isampuli ngcono**
Kwicandelo lekhemistri yohlalutyo, ukubaluleka kokulungiselela isampuli akunakugqithiswa. Esinye sezixhobo ezisebenzayo kule njongo yikhatriji ye-silica-based solid phase extraction (SPE). Yenzelwe ukwenza lula inkqubo yokukhupha, ezi khatriji zifumaneka kwiinkalo ezahlukeneyo, kubandakanya ubungakanani be-1, 3, 6, kunye ne-10 mL, ukuze zilungele iintlobo ngeentlobo zeesampuli kunye neentlobo zazo.
Iikholamu zethu ze-SPE ezisekwe kwi-silica ziyilwe ngononophelo ukuqinisekisa ukuba akukho mahluko mancinci ekusebenzeni. Oku kubalulekile kubaphandi kunye nabahlalutyi abaxhomekeke kwiziphumo ezihambelanayo. Iinkqubo zokuvelisa esizisebenzisayo azikhulisi nje kuphela ukubuyiswa kwee-target analytes, kodwa zikwaphucula neenkqubo zokucoca, okwenza kube lula ukufumana iisampulu ezicocekileyo kakhulu.
Phakathi kweendlela ezahlukeneyo ezikhoyo, iikholamu zethu zineentlobo ezintathu ezahlukeneyo zezigaba ezizinzileyo: i-C18 Endcapped Octadecyl, i-C18 Unendcapped Octadecyl, kunye ne-C8 Octyl. Iikholamu ze-C18 Endcapped Octadecyl zilungele zombini iikhompawundi ze-polar kunye ne-non-polar, zibonelela ngokugcinwa kunye nokukhetha okuhle kakhulu. Iikholamu ze-C18 Unendcapped Octadecyl zibonelela ngeenzuzo ezifanayo kodwa zilungele ngokukodwa ii-analytes ezingezizo ze-polar, ziqinisekisa ukusebenza kakuhle kuluhlu olubanzi lwezicelo. Kwabo basebenza ngeekhompawundi ze-polar ezincinci, iikholamu ze-C8 Octyl zibonelela ngendlela elungeleleneyo yokufikelela ekuhlukaniseni okusebenzayo ngelixa zigcina ukubuyiselwa okuphezulu.
Nokuba usebenza kumayeza, uvavanyo lokusingqongileyo, okanye ukhuseleko lokutya, iikholamu zethu ze-SPE ezisekwe kwi-silica zinokuhlangabezana neemfuno zakho zohlalutyo. Ngokukhetha ikholamu efanelekileyo yesicelo sakho esithile, unokuphucula ukusebenza kakuhle kwenkqubo yokulungiselela isampuli, okukhokelela kwiziphumo ezithembekileyo neziphindaphindwayo. Fumana amandla eekholamu ze-SPE ezisekwe kwi-silica namhlanje kwaye uphucule amandla akho ohlalutyo!